目的 研究五味子酚对东莨菪碱所致小鼠学习记忆能力的影响及机制。方法 动物随机分为正常对照组、东莨菪碱模型组、加兰他敏组和五味子酚药物组(低、中、高3个剂量组)。腹腔注射东莨菪碱制备小鼠痴呆模型, Morris水迷宫测试小鼠学习记忆能力,同时进行脑组织超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶及乙酰胆碱酯酶活性检测。结果 东莨菪碱注射后,小鼠学习记忆能力明显下降;五味子酚能明显缩短痴呆小鼠找到平台的潜伏期和游泳路程,同时提高痴呆小鼠脑组织超氧化物歧化酶和谷胱甘肽过氧化物酶活性,降低丙二醛含量,抑制乙酰胆碱酯酶活性,降低谷胱甘肽含量。结论 五味子酚对东莨菪碱诱导的痴呆小鼠的学习记忆能力具有明显改善作用,其机制可能与改善胆碱能系统功能、抗氧化能力有关。
Abstract
OBJECTIVE To study the effect and mechanism of schisanhenol on learning and memory acquired disorder induced by scopolamine. METHODS Mice were divided into six groups by random, control group, model group, galantamine group, and schisanhenol groups (low, middle, high dose). Scopolamine was injected intraperitoneally to induce learning and memory acquired disorder in mice. The learning and memory ability of mice were investigated by Morris maze, the activity of SOD, MDA, GSH-px, AChE in brain were measured. RESULTS The learning and memory ability of mice was severely impaired in scopolamine group. Schisanhenol significantly decreased the escaping latency and swimming distance of dementia mice in Morris water maze test induced by scopolamine, on the other hand, schisanhenol could increase the activity of SOD and GSH-px in brain, while the contents of MDA and the activity of AChE were depressed. CONCLUSION It is suggested that schisanhenol could improve the learning and memory capability by improve the cholinergic system and antioxidant ability.
关键词
五味子酚 /
东莨菪碱 /
学习记忆
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Key words
:schisanhenol /
scopolomine /
learning and memory
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中图分类号:
R965
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参考文献
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脚注
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基金
黑龙江省大学生创新创业训练重点项目(201311230010);齐齐哈尔医学院科研基金资助项目(QY2013B-01)
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